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This question came up during the last Instructor Renewal Class, & the response given was that there's not a requirement for DA vs SA, or safety on vs safety off.

From a training perspective, I have them use whatever condition the gun will be in while carrying & employing it defensively as this builds muscle memory (DA for a revolver; first shot in DA for a DA/SA semi-auto, then decock before the next engagement; safety engaged for a SA-only like a 1911, then reengage the safety before the next engagement; etc). The more repetitions an action is done, the more fluid it will be under stress.